Agdia Tomato Brown Rugose Fruit Virus (ToBRFV) Product Offerings
| Diagnostics | Description | Isolates Tested |
|---|---|---|
| Available now in reagent set and Pathoscreen® format! This is the most specific and sensitive ELISA method for the detection of ToBRFV. Used in a laboratory setting for cost-effective, high-throughput screening. Click here to read the press release. | Italy, Israel, Mexico, Germany | |
| Available now to purchase! Rapid, RNA-based, isothermal molecular method. Can be deployed to the field or used in a laboratory setting. Use as a stand-alone assay or use to directly confirm crude extracts of samples which test positive in ELISA. Click here to read the press release. | Italy, Israel, Mexico, U.S. | |
|
Available now to purchase! Field deployable, Cost-effective scouting tool that provides immediate on-site results. Higher specificity for and sensitivity to ToBRFV than the TMV ImmunoStrip®. Click here to read the press release. |
Italy, Israel, Mexico, Germany | |
| TMV ELISA | Cost effective, high-throughput screening. Reacts strongly with ToBRFV isolates via cross-reaction. Fully compatible with ISTA / ISF seed testing protocols. | Italy, Israel, Mexico |
| TMV ImmunoStrip® |
Field deployable scouting tool that provides immediate on-site results for detection of ToBRFV and other Tobamoviruses. Broadly reactive at genus level. Does not discriminate between Tobamovirus species. *Note: Italy isolate not tested due to lack of available material. Likely to be detected. |
Israel, Mexico* |
| Tobamovirus group PCR primers | High senstivity Tobamovirus detection. Confirmation of ToBRFV possible by sequencing through our Testing Services laboratory. | Italy, Israel, Mexico, U.S., South America |
ToBRFV Overview
Tomato brown rugose fruit virus (ToBRFV) is a Tobamovirus that was first observed infecting tomatoes in Israel in 2014. Since then, ToBRFV has also been detected in Europe, Asia and the Americas. It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable. Moreover, ToBRFV can overcome the Tm-22 gene in tomato that confers resistance to the Tobamoviruses Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). This virus is also known to cause significant losses in pepper. The rapid international spread of ToBRFV combined with its ability to break resistance genes is causing alarm within the seed production industry and tomato growers worldwide.
There are multiple diagnostic products and testing services available from Agdia for detection and identification of ToBRFV. Agdia's ToBRFV ELISA reliably detects ToBRFV with a high level of specificity and represents a logical and affordable high-throughput screening step in seed health testing programs. AmplifyRP® XRT for ToBRFV is a rapid, RNA-based, isothermal molecular method which can be be deployed to the field or used in a laboratory setting. Crude extracts of samples which test positive in the ELISA format can be directly tested with the AmplifyRP® assay for confirmation of results.
Additional ToBRFV Resources
EPPO Global virus distribution map and country list
ASTA ToBRFV Q&A Guide (Agdia TMV ImmunoStrip® as screening method)
Australia Dept. of Agriculture - ToBRFV Emergency Measures (Agdia TMV ELISA as approved method)
