Figure 1. Recombinase polymerase amplification (RPA) technology being used in conjunction with Agdia’s AmpliFire® isothermal fluorometer.
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Agdia, Inc. (Elkhart, IN) has announced an expanded field of use for their AmplifyRP® isothermal amplification technology that now allows detection of genetically modified traits and native plant genes.
AmplifyRP® employs recombinase polymerase amplification (RPA), an isothermal amplification technology first introduced in 2006, that provides target sensitivity and specificity comparable to that achieved with polymerase chain reaction (PCR). Since 2010, Agdia has been developing plant pathogen detection assays on the AmplifyRP® platform. Their products are marketed as AmplifyRP® XRT and AmplifyRP® Acceler8®, and include assays for detection of Xylella fastidiosa, Clavibacter sepedonicus, Plum pox virus and Candidatus Liberibacter asiaticus, in plant tissue. When paired with Agdia’s AmpliFire® isothermal fluorometer (Figure 1.), AmplifyRP® is a rapid, user-friendly system that can be implemented in the field or the lab by personnel with limited experience in molecular diagnostics.
The expanded utility of AmplifyRP® allows Agdia to increase their product offerings for the testing of genetically modified plants and plant products. Currently, Agdia’s products for this market are limited to serological platforms such as ELISA and ImmunoStrip®. In addition to this market expansion, Agdia considers pursuing new markets, such as plant species identification at seed and seedling stages of development.
“This is an exciting step forward in our continued effort to bring simple and accurate molecular testing solutions to our customers. We believe our experiences and successes in development of isothermal plant pathogen assays will provide a seamless path for detection of plant genes. We look forward to exploring new opportunities and helping our present and future clients realize the benefits this technology can bring to the table,” said Albert Vrient, Chief Operating Officer, Agdia, Inc.
About Agdia
A leading provider of diagnostic solutions for agriculture, Agdia, Inc. has been serving plant breeders, propagators, growers, universities, regulatory organizations and private testing laboratories since 1981. The company offers a comprehensive portfolio of validated, easy-to-use diagnostics for identifying plant pathogens, plant hormones and transgenic traits. Moreover, Agdia operates an ISO accredited, in-house, testing services laboratory. Agdia’s quality management system is ISO 9001:2015 certified and their Testing Services Laboratory is ISO 17025:2017 accredited. Visit the company’s website at www.agdia.com, e-mail info@agdia.com, phone 1-574-264-2615 (toll-free 800-622-4342) or fax 1-574-264-2153.
AmplifyRP®, Acceler8® and AmpliFire® are registered trademarks of Agdia, Inc.
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