AmplifyRP® isothermal chemistry and AmpliFire® portable fluorometer
Get clear results from crude extracts in 30 minutes or less with our robust isothermal DNA/RNA detection & identification platform.
AmplifyRP® is an isothermal chemistry utilizing RPA (recombinase polymerase amplification) to produce rapid and sensitive detection of nucleic acid targets via end point lateral flow analysis or real-time fluorescent based amplification.
BUY A TEST
New releases:
- HLVd (Hop latent viroid)
- Fusarium TR4
- TRV (Tobacco rattle virus)
- HSVd (Hop stunt viroid)
- GPGV (Grapevine pinot gris virus)
- Phytoplasma solani BN
- Phytoplasma vitis FD
- ToBRFV - Coming soon!
- GLRaV-3 (Grapevine leafroll-associated virus 3)
Click here to see all pathogen-specific kits available for purchase.
DEVELOP YOUR TEST
Work with us to develop a test on the AmplifyRP® platform. AmplifyRP® technology is compatible with most real-time PCR machines in addition to AmpliFire®.
Available targets include:
- Plant pathogens
- Native plant genes
- GMO trait targets
Try it yourself with a Discovery Kit, or contact us to brainstorm ideas. See below for an introduction to assay design considerations
DEPLOY YOUR TEST
Bring your test to the field with the AmpliFire®, a portable, 3-channel isothermal fluorometer.
AmpliFire® is chemistry agnostic, so you can use it with AmplifyRP®, LAMP or any other isothermal chemistry compatible with the included channels.
AmplifyRP® Discovery Kits: Designing Your Own Assay
Primer Design Considerations
- Amplicon total size < 250 base pairs
- Primer length 28 - 35 nucleotides
- Avoid homodimers & heterodimers
- Minimize degeneracy
- Acceler8® - 5’ biotin label required
- Similar melting temperature
Probe Design Considerations
- Probe length 46 - 52 nucleotides
- Avoid homodimers & heterodimers
- Avoid secondary structures
- Acceler8® - 5’ FAM label, internal THF (replaces any nucleotide), & 3’ blocker
- XRT internal fluorophore (replaces a “T” nucleotide, internal THF (replaces any nucleotide), internal quencher (replaces a “T” nucleotide), & 3’ blocker
Example Oligos
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